Nucleic acids can be amplified by various technologies and the
product can then be used in research or in diagnostics. The Polymerase
Chain Reaction (PCR) dominates the nucleic acid amplification market.
However, the requirement for thermocycling in PCR limits its application
in certain areas, such as field-testing and point-of-care diagnostics.
BioHelix has developed an isothermal DNA amplification system that
uses a helicase enzyme to unwind double stranded DNA (dsDNA), referred
to as Helicase-Dependent Amplification. In HDA reactions, duplex
DNA is separated into single strands by a helicase.
Compared with the other techniques, this amplification method is
closer to nature's method of performing DNA replication and it has
the following advantages:
1. Low cost for instrumentation because use of helicase to separate
DNA eliminates need for thermocycler.
2. Easy-to-use for assay development (using two primers).
3. Versatile platform: can amplify both DNA and RNA (with a reverse
transcriptase) and is compatible with multiple detection technologies.
4. High sensitivity and specificity.
5. BioHelix has IP surrounding the HDA technology.
Current products based on the HDA technology: