Detection using the BESt
The BESt cassette is comprised of 2 individual components:
1) Amplicon Cartridge - holds a single 0.2 ml thin
wall PCR tube that contains the reaction mixture, and the running
buffer is contained in a separate plastic bulb.
2) Detection Chamber - This unit contains a vertical-flow
test strip and holds the Amplicon Cartridge.
After the amplification reaction, Insert the tube
in an amplicon cartridge next to running buffer (Fig. 1, step 1).
Fold the cartridge in half and firmly close it (Fig. 1, step 2),
revealing an arrow on the top. Insert the closed cartridge halfway
in a detection chamber (Fig. 1, step 3), with the arrow pointing
the DNA test strip: do not use force to push it down.
Keep the assembled cassette upright and lower the handle and push
it into the chamber body to close the cassette (Fig. 1, step 4).
The handle will push the cartridge down, and lock into place
when closed completely (Fig. 1, step 5). This will trigger the release
of the amplicons, which are mixed with the running buffer at the
bottom of the cassette. The mixture then vertically flows up the
strip and the test results are shown as a colored line visible by
the naked eye.
Figure 1. Diagrams for detection procedure.