Download: Manual ; MSDS

Description:

IsoAmp® III Enzyme Mix is used to perform thermophilic Helicase-Dependent Amplification (tHDA), a novel method for isothermal amplification of nucleic acids. Like PCR, the tHDA reaction selectively amplifies a target sequence defined by two primers. However, unlike PCR, tHDA uses an enzyme called a helicase to separate DNA, rather than heat. This allows DNA amplification without the need for thermocycling. The tHDA reaction can also be coupled with reverse transcription for RNA analysis.

IsoAmp® III Enzyme Mix contains helicase, DNA polymerase and single-stranded DNA binding protein in a carefully optimized condition to support robust and specific amplification with reduced non-specific products due to primer-dimmer. In some cases, rapid amplification may be achieved by adding more IsoAmp® III Enzyme Mix into the reaction (unpublished observation).

Applications:

Used to amplify and detect short DNA sequences (80 - 120 bp) in tHDA reactions.

- Download Manual for IsoAmp III Universal tHDA Kit

Advantages:
Use of helicase to separate DNA eliminates need for thermocycler
Reactions can be performed at constant temperature.
Works with a variety of templates, including bacterial genomic DNA, viral DNA, plasmid DNA and cDNA.
As low as a single copy of target DNA can be amplified under optimized conditions.
Can be used for real-time quantitative or/and reverse transcription HDA.

Storage Conditions:

Quality Control:

Quality Assurance Statement:
Kit components were individually tested and confirmed free of endo- and exo-nuclease activity.

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