Download: MSDS

Description:

The T7 Gp4A Helicase exhibits a 5’ to 3’ helicase activity as well as primase activity. It is highly processive, traveling an average of 75 kb of single stranded DNA before dissociating.

SUPPLIED WITH:
10X Gp4A Reaction Buffer

Supplied by Users: dTTP

Source:

Reaction condition:

1X Gp4A Reaction Buffer:
35 mM Tris–acetate, pH 7.5
11 mM magnesium acetate
5 mM DTT
0.01% Triton X-100

Supplement with 2 mM dTTP.
Incubate at 25 - 37ºC.

NOTE:
The concentration of dTTP should be optimized for each specific application.
For the primase activity, supplement reactions with NTPs.

Storage Conditions:

Quality Control:

Quality Assurance Statement:
Thermostable Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity.