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Thermostable Helicase

 

Products - Helicases

Catalog #
Size
Concentration
Price
H0240S
20 µg
0.5 mg/ml
$568.00

Download: MSDS

   

Description:

   


Thermostable DNA Helicase is a DNA helicase that uses the energy generated by the hydrolysis of nucleoside triphosphates to break the hydrogen bonds linking the two strands together in duplex DNA. It is active in a temperature range of 45 to 65ºC.

SUPPLIED WITH:
10X Annealing Buffer II
100 mM MgSO4

Supplied by Users: ATP

     
     

Source:

   


Purified from an E. coli strain that overexpresses the helicase gene isolated from a thermophilic microorganism.
     
     

Reaction condition:

   


1X Annealing Buffer II:
10 mM KCl
20 mM Tris-HCl, pH 8.8 @ 25°C

Supplement with 4 mM MgSO4 and 3 mM ATP.
Incubate at 45 - 65ºC.

 
     

Storage Conditions:

   


Storage Temperature:

-20ºC

Concentration:
0.5 mg/ml

Storage Buffer:

20 mM Tris-HCl pH7.4
200 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
 
     

Quality Control:

   


Quality Assurance Statement:

Thermostable Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity.

     
   
   
 
 
 
   
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