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Thermostable Helicase

  

Products - Helicases
Catalog #
Size
Concentration
Price
H0240S
20 µg
0.5 mg/ml
$528.00

Download: MSDS
   

Description:

    


Thermostable DNA Helicase is a DNA helicase that uses the energy generated by the hydrolysis of nucleoside triphosphates to break the hydrogen bonds linking the two strands together in duplex DNA. It is active in a temperature range of 45 to 65ºC.

SUPPLIED WITH:
10X Annealing Buffer II
100 mM MgSO4

Supplied by Users: ATP
     
     

Source:

    


Purified from an E. coli strain that overexpresses the helicase gene isolated from a thermophilic microorganism.
     
     

Reaction condition:

    


1X Annealing Buffer II:
10 mM KCl
20 mM Tris-HCl, pH 8.8 @ 25°C

Supplement with 4 mM MgSO4 and 3 mM ATP.
Incubate at 45 - 65ºC.
 
     

Storage Conditions:

    


Storage Temperature:
-20ºC

Concentration:
0.5 mg/ml

Storage Buffer:
20 mM Tris-HCl pH7.4
200 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
 
     

Quality Control:

    


Quality Assurance Statement:
Thermostable Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity.
     
   
   
 
 
 
   
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