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ET SSB Plus

  

Products - Primer Navigator™ Series
Catalog #
Size
Concentration
Price
H0230S
100U
2U/µl
$198.00

Download: MSDS
   
Description:    


ET SSB Plus, (formally known as Primer Navigator™) is a mixture of thermostable RecA and thermostable SSB that is optimized for maximal enhancement of nucleic acid amplification, including HDA and PCR. SSB and RecA have been shown to improve the specificity and yield of RT-PCR, PCR and DNA sequencing (1-7). SSB proteins destabilize DNA secondary structure and increase the processivity of DNA polymerases (8) while RecA proteins bind to ssDNA and search for homology in dsDNA (9). SSBs have also been shown to enhance RecA function in ssDNA binding and strand transfer (10,11). ET SSB Plus has been developed to maximize their effect on difficult amplification reactions such as amplification of G+C rich region or multiplex PCR.
     
     
Reagent supplied:    


Solution A (50X)
     
     

Reaction condition:

    


Add 1 or 2U ET SSB Plus and 1 µl of Solution A in a 50 µl reaction.
     
     
     

Storage Conditions:

    


Concentration:
2U/µl

Storage Buffer:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.1% Triton X-100
50% Glycerol
pH7.5 @ 25ºC

Storage Temperature:
-20ºC

     
     

References:

    


  1. Shigemori, Y., Mikawa, T., Shibata, T. & Oishi, M. (2005) Nucleic Acids Res 33, e126.
  2. Villalva, C., Touriol, C., Seurat, P., Trempat, P., Delsol, G. & Brousset, P. (2001) Biotechniques 31, 81-3, 86.
  3. Schwarz, K., Hansen-Hagge, T. & Bartram, C. (1990) Nucleic Acids Res 18, 1079.
  4. Chou, Q. (1992) Nucleic Acids Res 20, 4371.
  5. Oshima, R. G. (1992) Biotechniques 13, 188.
  6. Rapley, R. (1994) Mol Biotechnol 2, 295-8.
  7. Olszewski, M., Rebala, K., Szczerkowska, Z. & Kur, J. (2005) Mol Cell Probes 19, 203-5.
  8. Myers, T. W. & Romano, L. J. (1988) J Biol Chem 263, 17006-15.
  9. Radding, C. M. (1991) J Biol Chem 266, 5355-8.
  10. Reddy, M. S., Vaze, M. B., Madhusudan, K. & Muniyappa, K. (2000) Biochemistry 39, 14250-62.
  11. West, S. C., Cassuto, E. & Howard-Flanders, P. (1982) Mol Gen Genet 186, 333-8.
 
   
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